<noscript id="gccgg"><kbd id="gccgg"></kbd></noscript>
  • <table id="gccgg"><kbd id="gccgg"></kbd></table>
  • 收藏本站    您好,欢迎光临上海懋康生物科技有限公司!
    登录  |   注册
    当前位置:首页> 新闻资讯> 新品发布 >MKbio Forskolin 佛司可林/毛喉素(最经典的腺苷酸环化酶活化剂)
    MKbio Forskolin 佛司可林/毛喉素(最经典的腺苷酸环化酶活化剂)
    时间:2018-01-25     作者:懋康原创     文章来源:懋康生物    

    Forskolin 佛司可林/毛喉素


    产品标签

    Forskolin (Fsk) 佛司可林;Adenylate cyclase (AC) 腺苷酸环化酶;cAMP 环磷酸腺苷;Neuron Differentiation 神经元分化;CAS NO:66575-29-9;


    景描述

    环磷酸腺苷(cAMP)由腺苷酸环化酶(Adenylate cyclase , AC)(一种12-跨膜域酶)产生,AC催化ATP转化生成3′,5′-cAMP和焦磷酸盐。cAMP是一种重要的信号载体,在细胞对激素,神经递体和其他细胞外信号的恰当的生理反应中是不可或缺的?;妨姿嵯佘眨╟AMP)由AC合成且经磷酸二酯酶(phosphodiesterases, PDEs)降解。局部cAMP信号通过靶向信号组分到亚细胞区室和组装信号复合物来获得。 


    产品描述

    佛司可林(Forskolin,CAS NO.:66575-29-9)是一种来源于印度彩叶植物毛喉鞘蕊花(Coleus forskohlii)的天然生成二萜。通过作用于催化亚基直接激活腺苷酸环化酶(AC),引起细胞内cAMP水平的提高[1-2]。在稳定转染且表达I型腺苷酸环化酶(AC1)的Sf9细胞,Forskolin与膜上的AC1结合(IC50=41nM)并活化其功能(EC50=0.5 μM,评估从ATP生成cAMP的激活实验)[3]。Forskolin以浓度依赖性的方式发挥平滑肌松弛剂和血管扩张剂的功能,同时影响细胞质钙离子水平和收缩成分的钙离子敏感性[4]。Forskolin能够与葡萄糖转运体和某些离子通道相互作用,可用来检测腺苷酸环化酶的表达、调控和G蛋白信号通路[5]。Forskolin用于几种分化方案中以增强神经元分化能力[6-7]。


    我司提供两种形式的佛司可林(Forskolin):

    一种是溶于DMSO的50mM储存液形式,对应货号是:MZ2301-50UL(~1mg),MZ2301-250UL(~5mg),直接用培养基稀释到所需工作浓度即可。操作简单方便。

    一种是冻干粉形式,对应货号是:MZ2301-50MG,MZ2301-100MG。需要先用DMSO或无水乙醇配制成储存液,再用培养基稀释到所需工作浓度使用。


    Forskolin通常用在各种类型的完整细胞或组织培养物中来提高cAMP水平,常用工作浓度为10-50μM。具体的最佳工作浓度请参考文献,或根据自身实验体系,培养细胞和组织类型,通过实验进行摸索和优化。



    产品应用(T淋巴细胞使用Forskolin抑制IL-2合成的应用示例)

    细胞类型:Jurkat cells (淋巴细胞样T细胞系)

    作用机制:Jurkat细胞能生成IL-2。正常生长情况,Jurkat细胞几乎没有或不生成IL-2。PMA,通过刺激PKC能活化T细胞并刺激低水平的IL-2生成。当Jurkat细胞经PMA和共刺激剂比如植物血凝素PHA处理,IL-2的生成明显得到加强。PHA本身能够激活低水平的T细胞活化和IL-2生成,通过非特异性结合到细胞表面受体复合物。T淋巴细胞中,Forskolin激活腺苷酸环化酶会抑制IL-2合成。Forskolin通过间接干扰磷脂酶C活化来发挥这一功能。Forskolin激活腺苷酸环化酶,产生cAMP,从而激活PKA。PKA能磷酸化PLC的丝氨酸残基,从而间接抑制IL-2生成。

    反应步骤:

    2.5 mL of Jurkat cells (1×106cells/mL) and 2.5 mL fresh culture medium (RPMI-1640 with 10% fetal calf serum containing 10 mL/L penicillin-streptomycin) were added to 25 cm2culture bottles. The following additions were made in duplicate.

    a. Control - no additions

    b. 1 μg/mL PHA + 10 ng/mL PMA [Add 10 μL of PHA stock solution (0.5 mg/mL PHA in filter-sterilized PBS) and 5 μL of PMA stock solution (10 μg/mL PMA in DMSO)]

    c. 0.2 mM Forskolin + 1 μg/mL PHA + 10 ng/mL PMA [Add 10 μL of PHA stock solution, 5 μL of PMA stock solution, and 10 μL of forskolin stock solution (100 mM forskolin in DMSO)

    After mixing well, the bottles were incubated at 37 °C for 24 hours. After centrifugation, the clarified broth was then tested for IL-2 production by ELISA assay. IL-2 production in the test cultures containing 0.2 mM forskolin was inhibited ≥50% compared to the test cultures containing only PHA and PMA. 


    产品特性

    1)CAS NO:66575-29-9

    2)化学名:5-(acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-(3R,4aR,5S,6S, 6aS,10S,10aR,10bS)-1H-naphtho[2,1-b]pyran-1-one

    3)同义名:Coleonol洋紫苏醇;NSC 375489;NSC 357088;L 75-1362B;HL 362;

    4)溶解性:溶于乙醇(~15 mg/ml),DMSO(~30 mg/ml),DMF(~30 mg/ml),几乎不溶于水

    5)分子式:C22H34O7

    6)分子量:410.50 g/mol

    7)纯度:>99%

    8)外观:白色结晶性粉末

    9)化学结构式:


    保存与运输方法

    保存:冻干粉-20oC避光保存,至少3年有效;DMSO储存液-20oC避光保存至少6个月有效;

    运输:冰袋运输。


    使用方法【源自文献,仅作参考】

    文献1,Rodriguez G et al.Forskolin-inducible cAMP pathway negatively regulates T-cell proliferation by uncoupling the interleukin-2 receptor complex. J Biol Chem. 2013 Mar 8;288(10):7137-46.PMID: 23341462

    体外研究:

    细胞类型(Cell type):Human leukemic cell line MT-2 cells

    药物配制(Preparation):Forskolinwas dissolved in DMSO to make stock solution.

    实验方法(Assay):MT-2 cells were seeded into 96-well plates at 5 × 104 cells per well. Cells were then pretreated for1 h with 1% DMSO (vehicle) or Fsk at 1, 5, 10, 25, 50, and 100 μM concentrations. The cells were stimulated with IL-2 as above and cultured for an additional 20 h at 37 °C. Control cells were treated with 1% DMSO for 20 h. During the final 4 h of incubation, the cells were pulsed with [3H]thymidine at a concentration of 0.5 μCi/200 μl. Cells were harvested onto fiberglass filters and analyzed using liquid scintillation counting.Fsk treatment inhibited the proliferation of MT-2 cells in a dose-dependent manner with an IC50 equal to~5 μM Fsk. 

    文献2,Matsumiya W et al. Forskolin modifies retinal vascular development in Mrp4-knockout mice. Invest Ophthalmol Vis Sci. 2012 Dec 7;53(13):8029-35. PMID:23154460

    体内研究:

    动物模型(Animal Model):C57BL/6J mice and Mrp4-knockout mice

    药物配制(Preparation):Forskolin was dissolved in dimethyl sulfoxide (DMSO) and then diluted.

    注射剂量(Dosages):Forskolin was dissolved in DMSO and injected intraperitoneally into neonatal mice at postnatal days 4 (P4) (1.0 μg/50 μL (0.3 mg/kg))and 5 (P5)(1.5 μg/50 μL (0.5 mg/kg)). Mice injected with DMSO served as the controls. The treated mice were euthanized at P6, and their retinas were isolated for whole-mount immunohistochemistry (IHC)

    给药途径(Administration):Intraperitoneally (i.p.) Injection


    订购信息:进口原料(广泛应用于全球各大实验室,纯度>99%),质量保证。小规格提供50μl(50mM in DMSO),按照10-50μM的工作浓度来算,可配制50ml-250ml工作液。 

    产品名称

    产品编号

    规格             

    CAS NO.       

    价格(元)      

    Forskolin (50mM in DMSO) 佛司可林    

    MZ2301-50UL        

    50μl

    66575-29-9

    150

    Forskolin (50mM in DMSO) 佛司可林

    MZ2301-250UL

    5×50μl

    66575-29-9

    520

    Forskolin (Powder) 佛司可林

    MZ2301-50MG

    50mg

    66575-29-9

    1480

    Forskolin (Powder) 佛司可林

    MZ2301-100MG

    100mg

    66575-29-9

    2540


    注意事项

    1)本品不是临床药物,只能用于科研用途,不能用于诊断或临床用途。

    2)为了您的安全和健康,请穿实验服并戴一次性手套操作。


    参考文献

    [1] Barber, R. and Goka, T.J. "Adenylate cyclase activity as a function of forskolin concentration." J. Cyclic Nucleotide Protein Phosphor. Res. 10: 23-29 (1985).

    [2] Laurenza, A., et al. "Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action?" Trends Pharm. Sci. 10: 442-447 (1989).

    [3] Robbins JD et al. Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J Med Chem. 1996 Jul 5;39(14):2745-52.

    [4] Abe, A. and Karaki, H.J. "Effect of forskolin on cytosolic Ca++ level and contraction in vascular smooth muscle." J. Pharmacol. Exp. Ther. 249: 895-900 (1989).

    [5] Insel PA et al. Forskolin as a tool for examining adenylyl cyclase expression, regulation, and G protein signaling. Cell Mol Neurobiol. 2003 Jun;23(3):305-14.

    [6] Palmer TD et al. The adult rat hippocampus contains primordial neural stem cells.Mol Cell Neurosci. 1997;8(6):389-404.

    [7] Son H et al. Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner. Neurosci Lett. 2001 Jul 27;308(1):37-40.


    相关产品

    货号

    名称

    规格          

    MZ2301-50UL     

    Forskolin (50mM in DMSO) 佛司可林

    50μl

    MZ2301-50MG

    Forskolin (Powder) 佛司可林

    50mg

    MZ2401-100UL

    PMA (TPA, 5mg/ml) 佛波肉荳蔻醋酸(5mg/ml)

    100μl

    MZ2402-5MG

    PMA (TPA, Powder) 佛波肉荳蔻醋酸(粉末,超纯)

    5mg

    MZ2403-1MG

    4α-PMA (4α-TPA) 4α-佛波肉荳蔻醋酸

    1mg

    MP6303-5MG

    Phytohemagglutinin-M (PHA-M) 植物血凝素-M(菜豆)    

    5mg

    MP6305-5MG

    Phytohemagglutinin-P (PHA-P) 植物血凝素-P(菜豆)

    5mg


     

     — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

     

     

    上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。


    K
    电话:021-54736159    传真:021-54736159    手机:18121428569
    邮箱: sales@maokangbio.com
    地址:上海市徐汇区龙吴路2888弄23号902室    
    版权所有:上海懋康生物科技有限公司
    97人妻精品全国免费视频
    <noscript id="gccgg"><kbd id="gccgg"></kbd></noscript>
  • <table id="gccgg"><kbd id="gccgg"></kbd></table>