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    当前位置: 首页> 产品中心> 细胞生物学 > 荧光探针与细胞染色 > MKBio BMPO (Spin trapping reagent) 自旋捕获剂
    MKBio BMPO (Spin trapping reagent) 自旋捕获剂
    目录号 MX4722-5MG 售价 1646.00元
    规格 5mg 运输温度 冰袋运输。
    其他名称 5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO; 保存温度 -20℃避光干燥保存,2年有效
    CAS号 387334-31-8 有效期 2年
    应用 自旋捕获剂 订购数量
    产品简介:

    BMPO (Spin trapping reagent)自旋捕获剂


    产品标签

    BMPO;DEPMPO;EMPO;Spin trapping reagent自旋捕获剂;电子顺磁共振波谱(EPR);CAS: 387334-31-8;


    产品信息

    产品名称

    产品编号

    CAS NO.            

    规格         

    价格(元)   

    BMPO (Spin trapping reagent)自旋捕获剂

    MX4722-5MG

    387334-31-8

    5mg

    1646

    BMPO (Spin trapping reagent)自旋捕获剂

    MX4722-10MG

    387334-31-8

    10mg

    2486

    BMPO (Spin trapping reagent)自旋捕获剂

    MX4722-50MG

    387334-31-8

    50mg

    8236


    产品描述

    BMPO(5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide)是一种新型高效且高稳定型的硝酮自旋捕获剂(Nitrone Spin Trapping Reagent),由美国威斯康星医学院Kalyanaraman教授的实验室团队开发,非常适合用于特异性检测和鉴定体内外形成的硫自由基、羟基自由基(?OH)和超氧阴离子自由基(?O2-),通过形成用电子顺磁共振波谱(EPR)可区分的加合物来测定[1-2]。


    BMPO具有以下特点:

    ①特异性高,半衰期长:其它的硝酮自旋捕获剂比如:DMPO,难以轻易区分超氧阴离子和羟基自由基,因产生的DMPO-超氧加合物(半衰期t?=45s)瞬间衰减产生DMPO-羟基加合物。与最近开发的自旋捕获剂:DEPMPO和EMPO类似,BMPO-超氧加合物不会衰减生成羟基加合物,但是,BMPO-超氧加合物的半衰期最长(t?=23min)。

    ②产品稳定性高:DEPMPO和EMPO是液体自旋捕获剂,通常被硝基氧杂质污染,且效期有限。BMPO是固体的环形硝酮,通过结晶以高度纯化的状态提供,能够保存更长的周期,且不用担心降解。

    ③更高的信噪比:BMPO衍生的加合物在各自的EPR光谱中具有更高的信噪比,使其更适合用于检测细胞悬浮液中的亚硫酸、羟基和甲基自由基。

    ④高水溶性:水溶性好,更利于水相体系自由基的研究,尤其是生物体系的自由基研究。


    产品特性

    1) CAS NO:387334-31-8

    2) 化学名:3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide

    3) 同义名:5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO;

    4) 分子式:C10H17NO3

    5) 分子量:199.2

    6) 纯度:≥98%

    7) 外观:结晶或结晶性粉末

    8) 溶解性:溶于水、PBS(pH 7.2, 10mg/ml)、DMSO(25mg/ml)、无水乙醇(25mg/ml)

    8)化学结构式:

    保存与运输方法

    保存:-20oC干燥保存,2年有效。 

    运输:冰袋运输。


    注意事项

    为了您的安全和健康,请穿实验服并戴一次性手套操作。


    参考文献

    1. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).

    2. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).


    应用示例(仅作参考)

    1.文献来源:Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, Eaton GR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J. 2013 Jul 16;105(2):338-42. doi: 10.1016/j.bpj.2013.06.005. PMID: 23870255; PMCID: PMC3714875. Editedby MKBIO


    ①黄嘌呤-黄嘌呤氧化酶体系内超氧阴离子(O2??)生成的测定:Typically, xanthine oxidase (0.04 U/mL) was added to pH ~7.4 sodium phosphate buffer (50 mM) containing DTPA (1 mM) and hypoxanthine (0.5–400 μM, final concentration) to achieve rates of O2?? formation that ranged from 0.1 to 6.0 μM/min. We estimated the superoxide formation rate by monitoring the SOD-inhibitable reduction of ferricytochrome c (80 μM) at room temperature. Spin trapping was performed by addition of 100 mM BMPO in pH ~7.4 phosphate-buffered saline (PBS; 50 mM) containing 1 mM DTPA to the solution of hypoxanthine and xanthine oxidase to achieve a final BMPO concentration of 50 mM in the reaction mixture. EPR spectra were recorded 10 min after mixing reagents. The half-life of BMPO-OOH at ambient temperature is reported to be ~23 min. Solutions for control experiments contained SOD (30 U/mL).

    Figure 2 Comparison of CW and rapid-scan spectra of BMPO-OOH in solution with a O2?? production rate of 0.1μM/min, recorded 10 min after mixing reagents. The O2?? was produced by a hypoxanthine/xanthine oxidase mixture. The concentration of BMPO-OOH is ~0.3μM. (A) CW spectrum obtained with 55 G sweep width, 0.75 G modulation amplitude, single 30 s scan, 15 ms conversion time, 10 ms time constant, and 20 mW (B1 = 170 mG) microwave power. (B) Deconvolved rapid-scan spectrum obtained with 55 G scan width, 51 kHz scan frequency, and 53 mW (B1 = 250 mG) microwave power. Segments consisting of 12 sinusoidal cycles were averaged 100 k times, with a total data acquisition time of ~30 s.


    2.文献来源:Wang, Z., Zhang, Y., Ju, E.et al. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors.Nat Commun 9,3334 (2018). https://doi.org/10.1038/s41467-018-05798-x 

    ①缺氧下的ESR测定(O2??,?OH):For O2?? detection, the pre-deoxidized PBS (pH 5.0, 25?mM) contained 25?mM BMPO, 20?μg?mL?1 MnO2@PtCo nanoflowers, 100?μM H2O2, 50% DMSO was prepared. After incubation of 5?min, ESR spectra were recorded. For ?OH detection, the pre-deoxidized PBS (pH 5.0, 25?mM) contained 25?mM BMPO, 20?μg?mL?1 MnO2@PtCo nanoflowers, 100?μM H2O2, 0.25?U?mL?1 SOD was prepared. After incubation of 5?min, ESR spectra were recorded. The following instrument settings were used for collecting ESR spectra: 1?G field modulation, 100?G scan range, and 20?mW microwave power.

    Fig f ESR spectra of BMPO/?OOH adducts from different groups in the hypoxic H2O2 (100?μM) condition upon addition of DMSO.g ESR spectra of BMPO/?OH adducts were collected from different samples in the hypoxic H2O2 (100?μM) condition upon addition of SOD. Data were presented as mean?±?s.d. (n=?5).


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